反应条件下苯丙氨酸解氨酶的活力稳定性,成果详细信息-中国科技论文在线

来自 : paper.edu.cn/scholar/paper/liq 发布时间：2021-03-24

By comparing the numbers of the consecutive reaction runs with a relative high PAL phenylalanine ammonia-lyase activity the stability of enzymic activity of PAL in the cells R glutinis NC06 was determined Various methods except reducing the physical severity of the reaction conditions such as high pH high ammonia and trans-cinnamic acid concentrations have been developed for stabilizing the PAL trader the bio-conversion condition The stability of the PAL in the cells can markedly be improved by omitting chlorideions CI- from the reaction mixture and using anaerobic condition cre- ated by N2-sparking during the reaction process The inclusion of glycerol especially sor-bitol in the reaction mixture appeared to significantly increase the stability of PAL in the cells The substitution of NH4 zCOa or NH4Ac for NIl3 in the reaction mixture and the immobilization of the PAL-containing cells by k-carrageenin also greatly contributed to the stability of the PAL activity Acombination of N2- sparking during the eactionprocess with inclusion of sorbitol and exclusion of CI- in the reaction mixture increased the number of the consecutive reaction runs with a high PAL activity more than 19 5mg trans-cinnamic acid per g cell wet weight per hour to 10-fold that of the controlwhich was carried out without any stabilizing means

Various methods except reducing the physical severity of the reaction conditions, such as high pH, high ammonia and trans-cinnamic acid concentrations, have...反应条件下苯丙氨酸解氨酶的活力稳定性，成果详细信息